Determination of Ascorbic Acid Content in the Drug "Asvitol" by Spectrophotometric and Chromatographic Methods

Purpose of research The aim of the work was to study the features of instrumental methods of qualitative and quantitative determination of ascorbic acid in a medicinal product.

Methods. For research, the drug "Asvitol" with a content of 200 mg of the main component in the form of ascorbic acid was selected as an object.

The main and auxiliary solutions were used in the work, in which distilled water obtained using an electric DEM 10 distiller acted as a medium, the pH of the solutions was determined on the pH meter of IPL-311. The process temperature was maintained using the UT-4300E ultrathermostat.

The ascorbic acid content was determined by the spectrophotometric method on the SHIMADZU VV-1800 spectrophotometer and by the method of high-performance liquid chromatography on the Agilent 1260 Infinity chromatograph. Results. The known methods of determination of ascorbic acid by the spectrophotometric method and by the method of highly effective liquid chromatography have been refined. The influence of the pH of the medium on the quantitative determination of ascorbic acid has been studied. The equation of the calibration dependence is obtained. The suitability of the Agilent 1260 Infinity chromatographic system for the analysis of the content of ascorbic acid in the preparation "Asvitol" was tested.

Conclusion. The conducted studies have shown the possibility of using both the spectrophotometric method and the method of high-performance liquid chromatography with a certain accuracy to determine the content of ascorbic acid in the drug "Asvitol".

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