Preview

Proceedings of the Southwest State University. Series: Engineering and Technology

Advanced search

Analysis of the Glycoside Composition of Calendula Extract

https://doi.org/10.21869/2223-1528-2024-14-1-175-191

Abstract

Purpose of research. The aim of the article is to comparatively analyse the glycoside (flavonoid) composition of liquid dosage forms containing calendula medicinalis as an initial raw material.

Methods. To achieve this goal, methods for extracting medicinal plant materials were studied, scientific literature on the research topic was analyzed, qualitative and quantitative analysis was carried out, and the obtained UV spectra were examined. Obtaining liquid dosage forms from Calendula officinalis flowers was carried out using a Soxhlet extractor and the maceration method. The optimal conditions for the extraction of glycosides were determined by varying the ratio of raw materials and extractant, as well as the concentration of the extractant. For quantitative assessment, the content of extractive substances in the resulting dosage forms was calculated. Confirmation of the presence of flavonoid compounds was carried out using UV spectroscopy. A comparative analysis of the content of flavonoids in liquid dosage forms based on calendula officinalis was carried out.

Results. As a result, liquid dosage forms were obtained from calendula officinalis flowers by two different methods, using different formulations, extraction was carried out by maceration and circulating extraction in a Soxlet apparatus, in ratios of 1:5 and 1:10, different concentrations of ethyl alcohol were also used (40%, 70%, 96%). Optimal extraction conditions were determined – for this raw material, the best indicators were achieved when obtaining the dosage form using the Soxlet extractor, in addition, according to the experiment, the concentration of ethyl alcohol, which extracts more target components, is 70%, the most effective ratio is 1:5.

Conclusion. The study examined changes in the glycosidic (flavonoid) composition in dosage forms prepared independently according to various recipes and drugs obtained in pharmaceutical production. The use of flavonoid extraction methods provides a great opportunity for further scientific research into the extraction of dietary supplements for the purpose of their production.

About the Authors

K. F. Yankiv
Southwest State University
Russian Federation

Karinе F. Yankiv, Candidate of Sciences (Pedagogical), Associate Professor of the Department of Physical Chemistry and Chemical Technology

50 Let Oktyabrya Str. 94, Kursk 305040



M. S. Evdokimova
Southwest State University
Russian Federation

Maria S. Evdokimova, Student of the Faculty of Natural Sciences

50 Let Oktyabrya Str. 94, Kursk 305040



M. I. Gorelova
Southwest State University
Russian Federation

Maria I. Gorelova, Student of the Faculty of Natural Sciences

Researcher ID: JOJ-6406-2023 

50 Let Oktyabrya Str. 94, Kursk 305040



References

1. Aljancic I., Vajs V., Menkovic N., Karadzic I., Juranic N., Milosavljevic S., Macura S. Flavones and sesquiterpene lactones from Achilleaatrata subsp. multifi da: antimicrobial activity. J. Nat. Prod., 1999. vol. 62(6), рр. 909–11.

2. Beresneva A. V., Yankiv K. F. [Extraction of flavonoids, saponins and tannins from horse chestnut seeds and their qualitative determination]. Fundamental'nye i prikladnye issledovaniya v oblasti khimii i ekologii – 2022. Sbornik nauchnykh statei Mezhdunarodnoi nauchno-prakticheskoi konferentsii studentov, aspirantov i molodykh uchenykh, posvyashchennoi 80-letiyu so dnya rozhdeniya professora F. F. Niyazi [Fundamental and applied research in the field of chemistry and ecology - 2022. Collection of scientific articles of the International scientific and practical conference of students, graduate students and young scientists, dedicated to the 80th anniversary of birth of Professor F. F. Niyazi]. Kursk, Universitetskaya kniga Publ., 2022, pp. 40–43. (In Russ.)

3. Tukhtayev Kh. R., Khamidov O. Zh., Sultanova R. Kh., Chinibekova N. K. Ekstrakt iz tsvetkov romashki na masle gor'kogo mindalya i poluchenie stabil'nykh emul'sii na ego osnove I [Extract from chalomile flowers in bitter almond oil and obtaining stable emulsions bazed on it]. Izvestiya vuzov. Khimiya i khimicheskaya tekhnologiya = Russian Journal of Chemistry and Chemical Technology, 2021, vol. 64, is. 7, рр. 61–67.

4. Kazub V. T., Kosheleva M. K., Rudobashta S. P. Kinetika izmel'cheniya rastitel'nogo syr'ya pri elektrorazryadnom ekstragirovanii [Kinetics of grinding of vegetable raw materials during electric discharge extraction]. Izvestiya vuzov. Khimiya i khimicheskaya tekhnologiya = Russian Journal of Chemistry and Chemical Technology, 2021, vol. 64, no. 6, no. 76–82.

5. Gorelova M. I., Yankiv K. F. [Flavonoids as biologically active compounds of maclura extract]. Fundamental'nye i prikladnye issledovaniya v oblasti khimii i ekologii – 2022. Sbornik nauchnykh statei Mezhdunarodnoi nauchno-prakticheskoi konferentsii studentov, aspirantov i molodykh uchenykh, posvyashchennoi 80-letiyu so dnya rozhdeniya professora F. F. Niyazi [Fundamental and applied research in the field of chemistry and ecology - 2022. Collection of scientific articles of the International scientific and practical conference of students, graduate students and young scientists, dedicated to the 80th anniversary of the birth of Professor F. F. Niyazi]. Kursk, Universitetskaya kniga Publ., 2022, pp. 51–54. (In Russ.)

6. Bandyukova V. A., Dzhumyrko S. F., Sergeeva N. V., Shinkarenko A. L. [Study of flavonoids of some plant species of the Asteraceae, borage and euonymus families]. Trudy pervogo vsesoyuznogo s"ezda farmatsevtov [Proceedings of the First All-Union Congress of Pharmacists]. Moscow, 1970, pp. 253–258. (In Russ.)

7. Ladygina E. Ya. Kalendula lekarstvennaya [Calendula officinalis]. Farmatsiya = Pharmacy, 1992, vol. 40, no. 4, pp. 84–86.

8. Omelchuk M. A. [Calendula officinalis - a source of raw materials for new drugs]. Sovremennye metody issledovaniya lekarstvennykh rastenii. Nauchnye trudy [Modern methods of researching medicinal plants. Scientific works]. Moscow, 1983, vol. XX, рр. 188–192. (In Russ.)

9. Fadeeva O. V. [Study of some representatives of the Asteraceae (Asteraceae) family for flavonoid content]. III Vsesoyuznyi s"ezd farmatsevtov. Tezisy dokladov [III All-Union Congress of Pharmacists: Proc. Report]. Kishinev, Timpul Publ., 1980, pp. 194–195.

10. Sharova O. V., Kurkin V. A. Flavonoidy tsvetkov kalenduly lekarstvennoi [Flavonoids of Calendula officinalis flowers]. Khimiya rastitel'nogo syr'ya = Chemistry of plant raw materials, 2007, no. 1, pp. 65–68.

11. Gosudarstvennaya farmakopeya RF [State Pharmacopoeia of the Russian Federation]. XIII ed. Moscow, FEMB Publ., 2015, vol. 1. 1470 p.

12. Evdokimova M. S., Yankiv K. F. [The main technological stages of obtaining extracts]. Fundamental'nye i prikladnye issledovaniya v oblasti khimii i ekologii – 2021. Sbornik nauchnykh statei Mezhdunarodnoi nauchno-prakticheskoi konferentsii studentov, aspirantov i molodykh uchenykh [Fundamental and applied research in the field of chemistry and ecology – 2021. Collection of scientific articles of the International Scientific and Practical Conference of Students, Postgraduate Students and Young Scientists]. Kursk, Universitetskaya kniga Publ., 2021, pp. 83–86. (In Russ.)

13. Kuznetsova M. A. Lekarstvennoe rastitel'noe syr'e i preparaty [Medicinal plant raw materials and preparations]. 2nd ed., reworked and additional. Moscow, Vysshaya shkola Publ., 2004. 191 p.

14. Ponomarev V. D. Ekstragirovanie lekarstvennogo syr'ya [Extraction of medicinal raw materials]. Moscow, Medicine Publ., 1976. 238 p.

15. Salova T. Yu., Gromova N. Yu. Teoreticheskie osnovy polucheniya biologicheski aktivnykh veshchestv iz rastitel'nogo i zhivotnogo syr'ya [Theoretical basis for obtaining biologically active substances from plant and animal raw materials]. Uspekhi sovremennogo estestvoznaniya = Advances of modern natural science, 2016, no. 3, рр. 39–43.

16. Leonova M. V., Klimochkin Yu. N. Ekstraktsionnye metody izgotovleniya lekarstvennykh sredstv iz rastitel'nogo syr'ya [Extraction methods for the production of medicines from plant materials]. Samara, Samara St. Technical Univ. Publ., 2012. 111 p.

17. Tyrkov A. G. Vydelenie i analiz biologicheski aktivnykh veshchestv [Isolation and analysis of biologically active substances]. Moscow, KNORUS Publ., Astrakhan, ASU Publ., 2016, рр. 69–83.

18. Mirovich V. M., Privalova E. G. Biologicheski aktivnye veshchestva rastenii (polisakharidy, efirnye masla, fenologlikozidy, kumariny, flavonoidy) [Biologically active substances of plants (polysaccharides, essential oils, phenologlycosides, coumarins, flavonoids)]. Irkutsk, IGMU Publ., 2018. 70 p.

19. Shinkarenko A. B., Bandyukova V. A., Kazakov A. L. Metody issledovaniya prirodnykh flavonoidov [Methods for studying natural flavonoids]. Pyatigorsk, Pyatigorsk farmac. Univ. Publ., 1977. 177 p.

20. Saushkina A. S., Karpenko V. A., Savchenko L. N., Muravyova D. A. Ispol'zovanie TSKh dlya identifikatsii biologicheski aktivnykh veshchestv v nekotorykh vidakh lekarstvennogo syr'ya [The use ofTLC for the identification of biologically active substances in some types of medicinal raw materials]. Sorbtsionnye i khromatograficheskie protsessy = Sorption and chromatographic processes, 2001, vol. 1, is. 5, pp. 902–905.


Review

For citations:


Yankiv K.F., Evdokimova M.S., Gorelova M.I. Analysis of the Glycoside Composition of Calendula Extract. Proceedings of the Southwest State University. Series: Engineering and Technology. 2024;14(1):175-191. (In Russ.) https://doi.org/10.21869/2223-1528-2024-14-1-175-191

Views: 265


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2223-1528 (Print)