Protective Properties of Ablated Cerium Oxide Nanoparticles for Cell Cultures under Conditions of Oxidative Stress under Ultraviolet and Ionizing Irradiation

Purpose. Study of the protective properties of ablated cerium oxide nanoparticles for cell cultures in an oxidative reaction under ultraviolet and ionizing irradiation.

Methods. Cerium dioxide nanoparticles with high antioxidant activity were obtained using laser ablation. Atomic force microscopy was used to characterize ablated cerium dioxide nanoparticles and cells incubated with cerium dioxide nanoparticles. The protective properties of cerium dioxide nanoparticles were studied after 0 (without incubation), 6and 24-hour incubation using the colorimetric MTT test method.

Results. Using laser ablation, cerium oxide nanoparticles with a maximum size of up to 50 nm were obtained. The resulting nanoparticles were incubated with BJ TERT cell lines for 6 and 24 hours. The samples were subjected to ultraviolet (UV) and ionizing irradiation in order to reveal the protective properties of CeO2 nanoparticles. Based on the results of the MTT test, it was found that incubation with cerium dioxide nanoparticles has a pronounced protective effect on the BJ TERT cell line. After UV irradiation, 6-hour and 24-hour incubation of cerium oxide nanoparticles with cell culture provides 15±5% and 20±5% more cell survival, respectively, than without particles. After ionizing radiation, the percentage of survival of cells incubated for 24 hours with cerium nanoparticles also increases by 20±5%.

Conclusion. This work shows that ablated cerium dioxide nanoparticles have a protective effect on healthy BJ TERT cells. The work shows that cerium oxide nanoparticles are promising antioxidants that can provide a protective effect for cell cultures from ultraviolet and ionizing radiation.

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